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Prof 

Syed, Khajamohiddin

Faculty: Faculty of Science, Agriculture and Engineering
Department: Biochemistry and Microbiology
ORCID: https://orcid.org/0000-0002-1497-3570
Research Interest(s): Bioinformatic, Genome-wide studies, Drug discovery, Communicable and non-communicable diseases, Sructural biology and evolution.
Active Community Engagement: South African Society for Microbiology (SASM) (membership number: 1518). Society of Biological Chemists (INDIA) (SBC, INDIA) (Life Membership number: 3218). South African Society for Bioinformatics (SASBi). South African Society for Biochemistry and Molecular Biology (SASBMB).
Biography: Prof Syed completed his M.Sc. in Biochemistry, receiving the gold medal, and a Ph.D. at Sri Krishnadevaraya University, Andhra Pradesh, India. After his doctoral studies he worked as postdoctoral research associate at the University of the Free State, Bloemfontein, South Africa (2006-2009); as a visiting scholar at the University of Cincinnati, Ohio, USA (2009-2013) and as a Lecturer and then Associate Professor at Central University of Technology (2013-2017). Currently he is an Associate Professor at the Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand, KwaDlangezwa, KwaZulu-Natal.
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20222
Author: Karpoormath, Rajshekhar ×
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    Lifestyles shape the cytochrome P450 repertoire of the bacterial phylum Proteobacteria
    2022
     | MDPI
    For the last six decades, cytochrome P450 monooxygenases (CYPs/P450s), heme thiolate proteins, have been under the spotlight due to their regio- and stereo-selective oxidation activities, which has led to the exploration of their applications in almost all known areas of biology. The availability of many genome sequences allows us to understand the evolution of P450s in different organisms, especially in the Bacteria domain. The phenomenon that “P450s play a key role in organisms’ adaptation vis a vis lifestyle of organisms impacts P450 content in their genome” was proposed based on studies on a handful of individual bacterial groups. To have conclusive evidence, one must analyze P450s and their role in secondary metabolism in species with diverse lifestyles but that belong to the same category. We selected species of the phylum Proteobacteria classes, Alpha, Beta, Gamma, Delta, and Epsilon, to address this research gap due to their diverse lifestyle and ancient nature. The study identified that the lifestyle of alpha-, beta-, gamma-, delta-, and epsilon-proteobacterial species profoundly affected P450 profiles in their genomes. The study determined that irrespective of the species associated with different proteobacterial classes, pathogenic species or species adapted to a simple lifestyle lost or had few P450s in their genomes. On the contrary, species with saprophytic or complex lifestyles had many P450s and secondary metabolite biosynthetic gene clusters. The study findings prove that the phenomenon mentioned above is factual, and there is no link between the number and diversity of P450s and the age of the bacteria.
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    An unprecedented number of cytochrome p450s are involved in secondary metabolism in Salinispora species
    2022
     | MDPI
    Cytochrome P450 monooxygenases (CYPs/P450s) are heme thiolate proteins present in species across the biological kingdoms. By virtue of their broad substrate promiscuity and regio- and stereo-selectivity, these enzymes enhance or attribute diversity to secondary metabolites. Actinomycetes species are well-known producers of secondary metabolites, especially Salinispora species. Despite the importance of P450s, a comprehensive comparative analysis of P450s and their role in secondary metabolism in Salinispora species is not reported. We therefore analyzed P450s in 126 strains from three different species Salinispora arenicola, S. pacifica, and S. tropica. The study revealed the presence of 2643 P450s that can be grouped into 45 families and 103 subfamilies. CYP107 and CYP125 families are conserved, and CYP105 and CYP107 families are bloomed (a P450 family with many members) across Salinispora species. Analysis of P450s that are part of secondary metabolite biosynthetic gene clusters (smBGCs) revealed Salinispora species have an unprecedented number of P450s (1236 P450s-47%) part of smBGCs compared to other bacterial species belonging to the genera Streptomyces (23%) and Mycobacterium (11%), phyla Cyanobacteria (8%) and Firmicutes (18%) and the classes Alphaproteobacteria (2%) and Gammaproteobacteria (18%). A peculiar characteristic of up to six P450s in smBGCs was observed in Salinispora species. Future characterization Salinispora species P450s and their smBGCs have the potential for discovering novel secondary metabolites.
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