Morphological and physiological screening of ethyl methanesulfonate treated sugarcane calli and plantlets for in vitro mannitol-induced osmotic stress tolerance

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dc.contributor.authorNtuli, Nontuthuko Rosemary
dc.contributor.authorDlamini, P.
dc.contributor.authorSchultz-Viljoen, T.
dc.coverage.conferenceissn
dc.date.accessioned2026-05-18T13:04:37Z
dc.date.available2026-05-18T13:04:37Z
dc.date.issued2020
dc.departmentNameBotany
dc.description.abstractSugarcane production in South Africa is limited by drought stress and its impact is expected to increase due to climate change. However, limited research is conducted to develop cultivars that are suitable for cultivation under water-stressed conditions. This study aimed to select, through morphological and physiological traits, the ethyl methanesulfonate treatment (s) that can produce calli and plantlets that are resistant to mannitol-induced osmotic stress. Sugarcane calli were exposed to ethyl methanesulfonate for 0.5, 1, 2, and 3 hours. To determine optimal selection lethal doses, calli were cultured on media containing 0, 150, 225 and 300 mM mannitol for further eight weeks under dark and light conditions. Incubation periods of half an hour and one hour induced genomic mutations without inhibiting callus growth and plant regeneration abilities. EMS-treated calli retained whiter, compact and with yellowish friable texture when compared with the control after the two-weeks recovery period post exposure to osmotic stress. Callus that was exposed for one hour was able to recover and regenerate plantlets at 225 and 407 mM mannitol stress. However, the two-hour incubation period and above resulted in stunted and albino plantlets. The LD50 and LD90 for selection were calculated as 225 and 407 mM mannitol. The EMS mutagenesis and in vitro selection for osmotic stress using mannitol can be used successfully to select sugarcane plantlets with better morphological and physiological responses to water stress in a very short time.
dc.facultyFaculty of Science, Agriculture and Engineering
dc.identifier.citationDlamini, P.J., Schultz-Viljoen, T. and Ntuli, N.R. 2020. Morphological and physiological screening of ethyl methanesulfonate treated sugarcane calli and plantlets for in vitro mannitol-induced osmotic stress tolerance. Plant Cell Biotechnol Mol Biol, 21(37-38), pp.107-120.
dc.identifier.issn0972-2025 (online)
dc.identifier.urihttp://hdl.handle.net/10530/59926
dc.inproceedingsissn
dc.issuenumber21 / 37-38
dc.keynoteissn
dc.language.isoEnglish
dc.pages107 - 120
dc.peerreviewedYes
dc.publisherSociety for Biology and Biotechnology
dc.subjectEthyl methanesulfonate mutagenesis
dc.subjectMannitol osmotic stress
dc.subjectSugarcane
dc.titleMorphological and physiological screening of ethyl methanesulfonate treated sugarcane calli and plantlets for in vitro mannitol-induced osmotic stress tolerance
dc.title.journalPlant Cell Biotechnology and Molecular Biology
dc.typeJournal Article
dspace.entity.typePublication
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