Antimicrobial resistance, enterotoxin and mec gene profiles of Staphylococcus aureus associated with beef-based protein sources from KwaZulu-Natal Province, South Africa

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dc.contributor.authorThwala, Thembeka
dc.contributor.authorMadoroba, Evelyn
dc.contributor.authorMaliehe, Tsolanku
dc.contributor.authorMagwedere, Kudakwashe
dc.contributor.authorButaye, Patrick
dc.contributor.authorBasson, Albertus K.
dc.coverageBasel, Switzerland
dc.coverage.conferenceissn
dc.date.accessioned2026-02-03T07:49:45Z
dc.date.available2026-02-03T07:49:45Z
dc.date.issued2022
dc.departmentNameBiochemistry and Microbiology
dc.description.abstractAnnually, approximately 23,000 cases of food poisoning by Staphylococcus aureus enterotoxins are reported worldwide. The aim of this study was to determine the occurrence and characterize S. aureus on beef and beef products in South Africa. Organ meats (n = 169), raw processed meat (n = 110), raw intact (n = 53), and ready-to-eat meats (n = 68) were obtained from 25 retail outlets. S. aureus was isolated and enumerated according to the ISO 6888-1 method. Identification of the strains was performed by MALDI-TOF MS. The antimicrobial resistance was determined using the disc diffusion test. The presence of methicillin-resistance genes and the staphylococcal enterotoxin genes was determined by PCR. Prevalence was low (13/400; CI 1.7–5) and all but one positive sample were from organ meats. Eight isolates were resistant to at least one antibiotic. Two isolates carried the mecC gene. All the isolates tested positive for seg, seh, sei, and sep, whilst 53.8% were positive for sea. None of the isolates was positive for ser, sej, seb, sec, or sed. The prevalence of S. aureus was low, with organ meats being the most contaminated. The presence of mecC-positive MRSA and of enterotoxins warrants further investigation and risk assessment.
dc.facultyFaculty of Science, Agriculture and Engineering
dc.format.preprintNo
dc.identifier.citationThwala, T., Madoroba, E., Maliehe, T.S., Magwedere, K., Basson, A.K. and Butaye, P., 2022. Antimicrobial resistance, enterotoxin and mec gene profiles of Staphylococcus aureus associated with beef-based protein sources from KwaZulu-Natal Province, South Africa. Microorganisms, 10(6), pp.1-13.
dc.identifier.issn2076-2607 (online)
dc.identifier.otherhttps://doi.org/10.3390/microorganisms10061211
dc.identifier.urihttp://hdl.handle.net/10530/58605
dc.inproceedingsissn
dc.issuenumber10 / 6
dc.keynoteissn
dc.language.isoen
dc.pages1 - 13
dc.peerreviewedYes
dc.publisherMDPI
dc.subjectMethicillin-resistant Staphylococcus aureus
dc.subjectStaphylococcal enterotoxin genes
dc.subjectAntimicrobial resistance
dc.subjectBeef-based products
dc.subjectS. aureus contamination
dc.subjectFood safety
dc.titleAntimicrobial resistance, enterotoxin and mec gene profiles of Staphylococcus aureus associated with beef-based protein sources from KwaZulu-Natal Province, South Africa
dc.title.journalMicroorganisms
dc.typeJournal Article
dspace.entity.typePublication
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