Saprophytic to pathogenic mycobacteria: loss of cytochrome p450s vis a vis their prominent involvement in natural metabolite biosynthesis

Simple item page
dc.contributor.authorZondo, Ntokozo Minenhle
dc.contributor.authorPadayachee, Tiara
dc.contributor.authorNelson, David R.
dc.contributor.authorSyed, Khajamohiddin
dc.coverageBasel, Switzerland
dc.coverage.conferenceissn
dc.date.accessioned2026-02-20T08:59:22Z
dc.date.available2026-02-20T08:59:22Z
dc.date.issued2022
dc.departmentNameBiochemistry and Microbiology
dc.description.abstractCytochrome P450 monooxygenases (P450s/CYPs) are ubiquitous enzymes with unique regio- and stereo-selective oxidation activities. Due to these properties, P450s play a key role in the biosynthesis of natural metabolites. Mycobacterial species are well-known producers of complex metabolites that help them survive in diverse ecological niches, including in the host. In this study, a comprehensive analysis of P450s and their role in natural metabolite synthesis in 2666 mycobacterial species was carried out. The study revealed the presence of 62,815 P450s that can be grouped into 182 P450 families and 345 subfamilies. Blooming (the presence of more than one copy of the same gene) and expansion (presence of the same gene in many species) were observed at the family and subfamily levels. CYP135 was the dominant family in mycobacterial species. The mycobacterial species have distinct P450 profiles, indicating that lifestyle impacts P450 content in their genome vis a vis P450s, playing a key role in organisms’ adaptation. Analysis of the P450 profile revealed a gradual loss of P450s from non-pathogenic to pathogenic mycobacteria. Pathogenic mycobacteria have more P450s in biosynthetic gene clusters that produce natural metabolites. This indicates that P450s are recruited for the biosynthesis of unique metabolites, thus helping these pathogens survive in their niches. This study is the first to analyze P450s and their role in natural metabolite synthesis in many mycobacterial species.
dc.facultyFaculty of Science, Agriculture and Engineering
dc.identifier.citationZondo, N.M., Padayachee, T., Nelson, D.R. and Syed, K., 2022. Saprophytic to pathogenic mycobacteria: Loss of cytochrome P450s vis a vis their prominent involvement in natural metabolite biosynthesis. International Journal of Molecular Sciences, 24(1), pp.1-14.
dc.identifier.issn422-0067 (online)
dc.identifier.issn1661-6596 (print)
dc.identifier.otherhttps://doi.org/10.3390/ijms24010149
dc.identifier.urihttp://hdl.handle.net/10530/58775
dc.inproceedingsissn
dc.issuenumber24 / 1
dc.keynoteissn
dc.language.isoen
dc.pages1 - 14
dc.peerreviewedYes
dc.publisherMDPI
dc.subjectMycobacterium tuberculosis
dc.subjectBiosynthetic gene clusters
dc.subjectNatural product
dc.subjectSecondary metabolism
dc.subjectCytochrome P450 monooxygenases
dc.subjectPathogens
dc.subjectEcological niches
dc.subjectAdaptation
dc.subjectComplex metabolites
dc.titleSaprophytic to pathogenic mycobacteria: loss of cytochrome p450s vis a vis their prominent involvement in natural metabolite biosynthesis
dc.title.journalInternational Journal of Molecular Sciences
dc.typeJournal Article
dspace.entity.typePublication
relation.isAuthorOfPublication89f4c77e-bc15-4300-bf81-aa80397b8a69
relation.isAuthorOfPublication.latestForDiscovery89f4c77e-bc15-4300-bf81-aa80397b8a69
relation.isOrgUnitOfPublication13ff708a-670d-4a85-bc85-e77baba2a8b1
relation.isOrgUnitOfPublication.latestForDiscovery13ff708a-670d-4a85-bc85-e77baba2a8b1
Files:
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Syed_Khajamohiddin_2022.pdf
Size:
1.66 MB
Format:
Adobe Portable Document Format

Syed_Khajamohiddin_2022.pdf (1.66 MB)

Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
5.24 KB
Format:
Item-specific license agreed upon to submission
Description:

license.txt (5.24 KB)

Download
Collections: Articles