Diversity and predicted functional roles of cultivable bacteria in vermicompost: bioprospecting for potential inoculum

Raimi, A. R.; Atanda, A. C.; Ezeokoli, O. T.; Madoroba, Evelyn; Adeleke, R. A.

Diversity and predicted functional roles of cultivable bacteria in vermicompost: bioprospecting for potential inoculum

dc.contributor.author	Raimi, A. R.
dc.contributor.author	Atanda, A. C.
dc.contributor.author	Ezeokoli, O. T.
dc.contributor.author	Madoroba, Evelyn
dc.contributor.author	Adeleke, R. A.
dc.coverage.conference	issn
dc.date.accessioned	2026-02-05T10:56:12Z
dc.date.available	2026-02-05T10:56:12Z
dc.date.issued	2022
dc.departmentName	Biochemistry and Microbiology
dc.description.abstract	Vermicompost (VC) harbours diverse microbes, including plant growth-promoting microorganisms (PGPM) that are beneficial for sustainable crop production. Hence, this study aimed to analyse bacterial diversity of VC samples as a first highthroughput screening step towards subsequent targeted isolation of potential bacterial inoculum candidates. To achieve this, bacterial communities in VC collected from five production farms were enriched in nutrient-rich media before highthroughput sequence (HTS) analysis of the partial 16S rRNA gene. HTS analysis revealed 572 amplicon sequence variants (ASVs) in all enriched VC samples. Firmicutes, Proteobacteria, Planctomycetes and Bacteroidetes were the most dominant phyla, while Lysinibacillus, Escherichia-Shigella, Bacillus, Pseudomonas, Clostridium sensu stricto 1, Morganella, Vibrio and Aeromonas were the predominant genera across the enriched VC. The presence of Clostridium sensu stricto 1, Escherichia-Shigella and Vibrio genera, which are potentially pathogenic species, suggests the need to improve vermicomposting efficiency and safety. Predicted functional profiling of the bacterial communities using PICRUSt2 showed abundance profiles of nitrogenases, phosphatases and sulfatases. In addition, the potential to produce siderophore, indole acetic acids (IAA) and phytohormone regulator 1-aminocyclopropane-1-carboxylate (ACC) were predicted. Lysinibacillus, Bacillus, Paenibacillus and Pseudomonas were major bacterial communities with potential plant growth-promoting traits and could serve as resources in bacterial inoculum production. The findings in this study provide insight into the community composition, abundance and the potential functional capability of cultivable bacterial species of enriched VC. This study also points to VC as a suitable source of potentially beneficial bacterial candidates for inoculum production
dc.faculty	Faculty of Science, Agriculture and Engineering
dc.format.preprint	No
dc.identifier.citation	Raimi, A.R., Atanda, A.C., Ezeokoli, O.T., Jooste, P.J., Madoroba, E. and Adeleke, R.A., 2022. Diversity and predicted functional roles of cultivable bacteria in vermicompost: bioprospecting for potential inoculum. Archives of Microbiology, 204(5), pp.1-13.
dc.identifier.issn	1432-072X (online)
dc.identifier.issn	0302-8933 (print)
dc.identifier.other	https://doi.org/10.1007/s00203-022-02864-3
dc.identifier.uri	http://hdl.handle.net/10530/58633
dc.inproceedings	issn
dc.issuenumber	204 / 5
dc.keynote	issn
dc.language.iso	en
dc.pages	1 - 13
dc.peerreviewed	Yes
dc.publisher	Springer Nature
dc.subject	Bacterial communities
dc.subject	Vermicompost
dc.subject	Plant growth-promoting bacteria
dc.subject	Biofertiliser
dc.subject	Enzyme-coding genes
dc.subject	High-throughput sequencing
dc.title	Diversity and predicted functional roles of cultivable bacteria in vermicompost: bioprospecting for potential inoculum
dc.title.journal	Archives of Microbiology
dc.type	Journal Article
dspace.entity.type	Publication
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