2 results
Search Results
Now showing 1 - 2 of 2
- The antioxidant, cholinergic, monoaminergic, and purinergic activities of flavonoid-rich extract from Dalbergiella welwitschii leaf (FEDW) were investigated on oxidative testicular injury (ex vivo) due to the local report on the use of this plant as anti-testicular injury. Flavonoid extract was obtained from FEDW using a standard procedure. Five male albino rats were used, testes harvested and incubated with FeSO4 for accessing the cholinergic, monoaminergic, and purinergic activities of the FEDW (ex vivo). Testicular tissues incubated with FeSO4 demonstrated a significant decrease in antioxidant biomarkers, arginase, ATPase, ENTPDase, 5ʹ-nucleotidase, and PDE-5 activities, as well as Zn and sialic acid levels with an upsurge in malondialdehyde (MDA), and NO levels, myeloperoxidase, cholinesterases, monoamine oxidase (MAO), and angiotensin-converting enzyme (ACE) activities. Treatment of testicular tissues incubated with FeSO4 via different concentrations of FEDW significantly increased the activities of antioxidant, arginase, ATPase, E-NTPDase, 5ʹ-nucleotidase, phosphodiesterase-5 (PDE-5), as well as Zn and sialic acid levels with a significant decrease in MDA, nitric oxide (NO), myeloperoxidase, cholinesterases, MAO, and ACE levels. Molecular docking revealed the molecular interactions of cyclooxygenase-2 (COX-2) with ellagic acid, piperine, and caffeine with piperine and caffeine obeyed the druggability and pharmacokinetic. These findings point to FEDW as a possible potential for the treatment of oxidative testicular injury.
- 1
- 0
- 0
- Breast cancer remains the most frequently diagnosed cancer worldwide. Several drugs have been identified and found to be active against the pathogenesis of breast cancer, however, the uniqueness each tumor cell exhibit remains a major issue. The difference in the phenotypes of tumor cells and the high metastatic activity they exhibit have heightened the incurability of breast cancer. It is completely necessary to continue to discover potentially active molecular species that can effectively treat all breast cancer types. To this end, this study employs a biomolecular simulation protocol to screen the compounds of Hibiscus sabdariffa and identify compounds that can bind to and potentially inhibit the activities of selected target enzymes and receptors. Using a molecular docking approach, several compounds were identified in each protein category and reported herein. The top five compounds had docking scores ranging from −9.52 to −11.79 kcal mol−1 on Human Epidermal Growth Factor Receptor 2 (HER2), −11.09 to −13.63 kcal mol−1 on Epidermal Growth Factor Receptor (EGFR), −11.07 to −14.03 kcal mol−1 on Progesterone receptor (PR), −11.53 to −14.71 on Phosphatidylinositol 3-kinase (PI3K) and −8.16 to −9.31 on Estrogen receptor (ER). Subsequently, MM/GBSA protocol was employed to rescore the docked complexes and calculate the binding energy. Further ADMET screening on the top-scoring compounds returned good pharmacokinetic behaviors. Conclusively, the results showed that these compounds could be explored as natural alternatives to available drugs, however, validatory tests are recommended.
- 1
- 3
- 0