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- Lagenaria siceraria is one of the most important cucurbitaceous vegetables due to its prolific bearing habit, its edibility as a cooked vegetable, and its low cost of cultivation. The objective of this study was to evaluate variation in the morpho-agronomic traits among selected landraces and their F1 populations. The landraces were crossed based on the North Carolina II genetic design to develop F1 populations. The twelve F1 populations along with seven parental landraces were grown in a randomized complete block design with three replications. Significant differences (p < 0.05) were observed among quantitative traits suggesting considerable genetic variability. The genotypes displayed significant variation in most qualitative traits of fruits and seeds. The first five principal components of quantitative traits among the evaluated 19 genotypes contributed 74.84% of the variability. The biplot and dendrogram clustered the genotypes into five clusters according to their vegetative, fruit, and seed traits. The highest value for the broad-sense heritability estimate was recorded for days to edible harvest maturity trait. The F1 progenies were more variable than the landraces and can therefore be used for further Lagenaria siceraria genetic improvement.
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- 2019| Gaurav PublicationsCorchorus olitorius is an underutilized, but nutrient-rich leafy vegetable. However, the nutritive value and micronutrient bioavailability of this vegetable has not been well researched in South Africa. Therefore, this study aimed at using a novel breeding technique, that is, utilization of colchicine treatment on mineral content of C. olitorius leaves. C. olitorius seeds were treated with 0, 0.025, 0.05, 0.075 and 0.1 g/l of colchicine for 2, 4, 6, 8 and 10 h. Treated seeds were sown in pots and laid out in a completely randomized design with three replications. Tender leaves were harvested at 49 days after planting for mineral content analysis using atomic absorption spectrophotometry. Data were subjected to ANOVA, principal component analysis and cluster analysis. Treatment with 0.025 g/l of colchicine for 2 h resulted in the highest nitrogen, phosphorus, potassium, calcium, magnesium, aluminium and iron content in C. olitorius leaves. Other studied leaf minerals decreased with an increase in colchicine concentration and prolonged treatment duration. All minerals were correlated positively with each other, but sodium, manganese and copper had an exclusive association with one another. Again, all minerals were positively associated with first principal component, while Na, Mn and Cu were associated with the second principal component. Biplot and dendrogram grouped treatments mainly according to treatment duration. The study also demonstrated high levels of variation among the colchicine treatments in the nutritional traits evaluated. This implies that colchicine has the potential to induce genetic variability which can be used for future C. olitorius crop improvement.
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